|
| Status |
Public on Mar 16, 2016 |
| Title |
pES10 h-G1 rep1 |
| Sample type |
genomic |
| |
|
| Source name |
Haploid pES10 cells in G1 (1c) rep1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: h-pES10
cell type: Haploid pES10 cells
cell cycle phase: G1 (1c)
passage: 15
differentiation status: Undifferentiated
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated (NucleoSpin Tissue Kit, MACHEREY-NAGEL) and bisulfite converted (EZ DNA Methylation Kit, Zymo Research) according to the manufacturer's protocol
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
HumanMethylation450 BeadChips were scanned with Illumina's iScan System
|
| Description |
100973330114_R01C01
|
| Data processing |
IDAT files were processed and normalized by subset-quantile within array normalization (SWAN) and adjusted for batch effects using the R package ChAMP (version 1.4.0)
|
| |
|
| Submission date |
Jul 28, 2015 |
| Last update date |
Mar 16, 2016 |
| Contact name |
Ido Sagi |
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
|
| Street address |
Givat Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE71457 |
Derivation and differentiation of haploid human embryonic stem cells [methylation array] |
| GSE71458 |
Derivation and differentiation of haploid human embryonic stem cells |
|
