|
| Status |
Public on Jun 14, 2007 |
| Title |
Substantia nigra normal rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Postmortem human substantia nigra
|
| Organism |
Homo sapiens |
| Characteristics |
Old Control
female
|
| Growth protocol |
Brain tissue was removed with a Post-Mortem Interval (PMI) of 9.03±2.2 hr for Control samples and 7.05±1.25 hr for the PD samples, subsequently sectioned and frozen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol lysis extraction of total RNA was performed on an M48 BioRobot with a Quiagen Megatrack Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for >16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 7G Scanner.
|
| Description |
Gene expression data of substantia nigra from postmortem brain of normal controls
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 25, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jarlath M.H. ffrench-Mullen |
| E-mail(s) |
[email protected]
|
| Phone |
1-240-364-7622
|
| Fax |
1-240-364-7603
|
| Organization name |
GeneLogic, Inc.
|
| Department |
CNS Program
|
| Street address |
610 Professional Drive
|
| City |
Gaithsburg |
| State/province |
MD |
| ZIP/Postal code |
20879 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7621 |
Expression data of substantia nigra from postmortem human brain of Parkinson's disease patients (PD) |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
