NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM189781 Query DataSets for GSM189781
Status Public on May 05, 2009
Title Healthy Twin 25a
Sample type RNA
 
Source name Intestinal mucosa biopsy from healthy human individuals
Organism Homo sapiens
Characteristics other: pair 25
gender: female
age: 22
genotype/variation: dizygotic twin
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy mini extraction was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared using Message Amp II-Biotin (Ambion) and further processed using the standard Affymetrix protocol (Affymetrix manual 2005)
 
Hybridization protocol Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133 2.0 Plus Array (Affymetrix GeneChipHybridization Oven 640). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3030
Description Gene expression data from healthy human twins (mucosal biopsies), female, age: 22a
Data processing Sample data was processed using GCOS 1.3.0 (Affymetrix default settings) and normalized using GCRMA
 
Submission date May 16, 2007
Last update date Aug 28, 2018
Contact name Robert Haesler
E-mail(s) [email protected]
Phone +49 (0) 431 597 5754
Organization name University Clinic of Schleswig Holstein
Department IKMB / 1st Dept. of Medicine
Lab Functional Genomics Platform
Street address Schittenhelmstr. 12
City Kiel
ZIP/Postal code 24105
Country Germany
 
Platform ID GPL570
Series (1)
GSE7821 Expression data from human intestinal biopsies
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE GCOS 1.3 / GCRMA calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 9.336942116 P 0.000218889
1053_at 6.740038968 P 0.002616955
117_at 2.459608013 A 0.127605543
121_at 3.267809796 P 0.000959383
1255_g_at 2.178471608 A 0.892699063
1294_at 3.668415875 P 0.002616955
1316_at 2.697370835 A 0.081196122
1320_at 2.614600399 A 0.5
1405_i_at 9.442811189 P 0.000941148
1431_at 2.432631368 A 0.267462611
1438_at 6.105424758 P 0.000959383
1487_at 8.199553704 P 0.004862762
1494_f_at 2.226808195 A 0.218983293
1552256_a_at 7.205101965 P 0.000732422
1552257_a_at 9.433520648 P 0.000732422
1552258_at 2.383231185 A 0.5
1552261_at 2.670238164 A 0.423828125
1552263_at 4.736894915 P 0.00195313
1552264_a_at 7.328027 P 0.000244141
1552266_at 2.750541245 A 0.334473014

Total number of rows: 54675

Table truncated, full table size 1936 Kbytes.




Supplementary file Size Download File type/resource
GSM189781.CEL.gz 3.7 Mb (ftp)(http) CEL
GSM189781.CHP.gz 324.5 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap