|
| Status |
Public on Jun 12, 2017 |
| Title |
30°C eWAT,biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
epididymal adipose tissue from mice exposed to 30°C
|
| Organism |
Mus musculus |
| Characteristics |
tissue: epididymal adipose tissue
|
| Treatment protocol |
Epididymal adipose tissue was washed by cold RNase-free normal saline, quickly dissected and put in liquid nitrogen when sacrificing the mice. Tissues were stored in -80°C until RNA extraction.
|
| Growth protocol |
Epididymal adipose tissue from mice exposed to 30°C, 4°C, -10°C and -20°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All samples were prepared using Trizol. Total RNA was isolated using the RNeasy Mini Kit (QIAGEN), cDNA synthesis and amplification was performed using the WT cDNA synthesis and amplification kit (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA by using Ambion® WT Expression Kit.
|
| |
|
| Hybridization protocol |
Following labeling, 5.5 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip Mouse Exon 1.0 in Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
Gene expression data from mice epididymal adipose tissue exposed to 30°C
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA) using Affymetrix Expression Console default analysis settings and global scaling as normalization method. Normalized signal intensities were transformed to the log base 2 scale.
|
| |
|
| Submission date |
Oct 15, 2015 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Xiaoyan Yang |
| Organization name |
Shandong University Qilu Hospital
|
| Street address |
107 West Wenhua Road
|
| City |
Jinan |
| ZIP/Postal code |
250012 |
| Country |
China |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE74062 |
Expression data from mice epididymal adipose tissue |
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