|
| Status |
Public on Nov 07, 2015 |
| Title |
Med22RNAi_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
1 day old adult testes
|
| Organism |
Drosophila melanogaster |
| Characteristics |
developmental stage: 1 day old adult
tissue: testis
genotype: bam-gal4; Med22RNAi
|
| Treatment protocol |
testes without seminal vesicle and accessory glands were dissected in PBS for 30 minutes and immediately frozen in liquid nitrogen before transferred to -80°C. Total RNA was extracted using TRIzol (Invitrogen). RNA quality was assessed by checking rRNA integrity using Bioanalyzer. ~250ngs of total RNA was processed for each replicate.
|
| Growth protocol |
Crosses and experimental flies were grown on cornmeal and molasses media at 25 °C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from ~250ng total RNA (GeneChip 3' IVT expression kit)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000-7G
|
| Data processing |
Data were background adjusted and normalized using R program (v3.0.2) with the BioConductor package GCRMA.
|
| |
|
| Submission date |
Nov 06, 2015 |
| Last update date |
Nov 07, 2015 |
| Contact name |
Chenggang Lu |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford University
|
| Street address |
279 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE74784 |
Gene expression of fly testes with Med22 knock down |
|
