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Sample GSM1934400 Query DataSets for GSM1934400
Status Public on Dec 16, 2015
Title 2010 FluCenter TIV subject ID 107 at D7 post-vaccination
Sample type RNA
 
Source name Peripheral blood mononuclear cells of subjects vaccinated with Influenza vaccine at day 0 (before vaccination), and days 3 and 7 post-vaccination.
Organism Homo sapiens
Characteristics tissue: blood
cell type: mononuclear cells
time point: D7
subject id: 107
Extracted molecule total RNA
Extraction protocol Following PMBC isolation from CPT, 2 x 10^6 cells were lysed in 1 ml of TRIzol and stored at -80C (Cat# 15596-026; Invitrogen Life Technologies). After all time points were collected for a subject, the samples were thawed, and the RNA isolation proceeded according to the manufacturer’s protocol.
Label biotin
Label protocol Total RNA sample quality was evaluated by spectrophotometer to determine quantity, protein contamination and organic solvent contamination, and an Agilent 2100 Bioanalyzer was used to check for RNA degradation. Two-round in vitro transcription amplification and labeling was performed starting with 50 ng intact, total RNA per sample, following the Affymetrix protocol.
 
Hybridization protocol Hybridization was performed on Human U133 Plus 2.0 Arrays (using GeneTitan platform, Affymetrix, or individual cartridges) for 16 h at 45 oC, and 60 r.p.m. in a Hybridization Oven 640 (Affymetrix), slides were washed and stained with a Fluidics Station 450 (Affymetrix).
Scan protocol Scanning was performed on a 7th generation GeneChip Scanner 3000, and the Affymetrix software was used to perform image analysis and generate raw intensity data.
Data processing RMA normalization was performed using Expression Console software.
 
Submission date Nov 09, 2015
Last update date Feb 25, 2021
Contact name Helder I. Nakaya
E-mail(s) [email protected]
Phone +551126481130
Organization name Universidade de São Paulo
Lab csbiology.com
Street address AVENIDA PROFESSOR LINEU PRESTES, 580, Block 17
City SÃO PAULO
State/province SÃO PAULO
ZIP/Postal code 05508000
Country Brazil
 
Platform ID GPL13158
Series (2)
GSE74815 Time Course of Adults Vaccinated with Influenza TIV Vaccine during 2010/11 Flu Season (FluCenter cohort)
GSE74817 Time Course of Adults Vaccinated with Influenza TIV Vaccine 2009-2012

Data table header descriptions
ID_REF
VALUE RMA Signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 6.22006
AFFX-BioB-M_at 7.28111
AFFX-BioB-3_at 6.75967
AFFX-BioC-5_at 7.73876
AFFX-BioC-3_at 8.16339
AFFX-BioDn-5_at 9.65282
AFFX-BioDn-3_at 10.5391
AFFX-CreX-5_at 12.1619
AFFX-CreX-3_at 12.4329
AFFX-DapX-5_at 7.95735
AFFX-DapX-M_at 8.87147
AFFX-DapX-3_at 9.14776
AFFX-LysX-5_at 4.79603
AFFX-LysX-M_at 5.36469
AFFX-LysX-3_at 6.28567
AFFX-PheX-5_at 5.59088
AFFX-PheX-M_at 6.3372
AFFX-PheX-3_at 5.63055
AFFX-ThrX-5_at 5.78135
AFFX-ThrX-M_at 6.20438

Total number of rows: 54715

Table truncated, full table size 1159 Kbytes.




Supplementary file Size Download File type/resource
GSM1934400_FC10_9_107_D7.CEL.gz 2.2 Mb (ftp)(http) CEL
GSM1934400_FC10_9_107_D7.rma.chp.gz 378.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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