|
| Status |
Public on Mar 16, 2016 |
| Title |
HuES53 |
| Sample type |
SRA |
| |
|
| Source name |
Diploid ES cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HuES53
passage: <20
differentiation status: Undifferentiated
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was isolated using the Total RNA Purification Micro Kit (Norgen). For RNA sequencing analysis, poly(A) pull-down was used to enrich mRNAs from total RNA samples (200 ng–1 µg per sample, RIN > 8)
Libraries were prepared using the Illumina TruSeq RNA prep kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Reads were mapped to the human reference genome (NCBI/build37.2) using Tophat (version 2.0.4)
Fragments per kilobase of transcript per million mapped reads (FPKM) values were calculated using Cufflinks (version 2.0.2)
Genome_build: NCBI/build 37.2
Supplementary_files_format_and_content: Tab-delimited text file includes FPKM values for each sample
|
| |
|
| Submission date |
Nov 12, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ido Sagi |
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
|
| Street address |
Givat Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE71458 |
Derivation and differentiation of haploid human embryonic stem cells |
| GSE74953 |
Derivation and differentiation of haploid human embryonic stem cells [RNA-Seq 2] |
|
| Relations |
| BioSample |
SAMN04262532 |
| SRA |
SRX1429048 |
