|
| Status |
Public on Dec 01, 2015 |
| Title |
NOR3, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
mRNA profiling data from the kidney of SD rat fed with 13 g/d of a normal calcium diet.
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
gender: male
disease state: control
diet: normal calcium diet
tissue: kidney
|
| Treatment protocol |
The colony of GHS rats were created by the selective breeding of male and female SD rats with the highest 24 hour urine excretions (doi: 10.3390/ijms160716313). By the 28th generation, GHS rats stably excreted significantly higher levels of urine calcium than wild-type normal SD rats.
|
| Growth protocol |
all rats were acclimated at 23℃ on a 12 h light/dark and fed with 13 g/d of a normal calcium diet in the SPF level animal house.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Fibrous Tissue kit (Qiagen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
The Affymetrix Whole Transcript Sense Target Labeling Assay (Affymetrix) is used to create biotin – labeled sense -strand cDNA targets for hybridization to the Gene 1.0 ST Arrays.
|
| |
|
| Hybridization protocol |
Hybridization cocktail for a single probe array contained 2.5 ?g of fragmented cRNA, 50 pM Control Oligonucleotide B2 (Affymetrix), 20X Eukaryotic Hybridization Controls (1.5 pM bioB, 5 pM bioC, 25 pM bioD, 100 pM cre) (Affymetrix), 7% DMSO, and 1X Hybridization Buffer. GeneChip Rat Gene 1.0 ST Array– Affymetrix was hybridized at 45?C for 18 hours in the GeneChip Hybridization Oven 640 (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Male SD rats with a body weight of 250-280g, and fed with 13 g/d of a normal calcium diet (1.2% calcium, 0.65% phosphorus, 0.43% chloride, 0.4% sodium, and 0.24% magnesium per 1 g of food)
|
| Data processing |
The data were analyzed with GeneSpring version 7.3 using RMA normalization, then normalized to median of all samples.
|
| |
|
| Submission date |
Dec 01, 2015 |
| Last update date |
Dec 01, 2015 |
| Contact name |
Yuchao LU |
| E-mail(s) |
[email protected]
|
| Organization name |
Tongji Hospital of Tongji medical college, Huazhong University of Science &Technology
|
| Department |
Urology
|
| Street address |
1095# Jiefang Avenue
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430030 |
| Country |
China |
| |
|
| Platform ID |
GPL6247 |
| Series (2) |
| GSE75542 |
Analysis of altered mRNA expression profiles in the genetic hypercalciuric stone-forming (GHS) rat compared to normal SD rat |
| GSE75543 |
miRNA and mRNA profiles in the genetic hypercalciuric stone-forming (GHS) rat compared to normal SD rat |
|
