|
| Status |
Public on Dec 04, 2015 |
| Title |
CP-Onset-2 |
| Sample type |
RNA |
| |
|
| Source name |
CP-Onset
|
| Organism |
Mus musculus |
| Characteristics |
strain: SJL mice (Charles River, Barcelona, Spain)
gender: female
age: 6–8 weeks of age adult
mouse status: experimental autoimmune encephalomyelitis (EAE)
eae phase: Onset
tissue: choroid plexus (CP)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
BeadStudio software (Illumina Inc., San Diego, CA, USA) was read into R/Bioconductor and normalized using quantile normalization. A linear model was applied to the normalized data using Limma package in R/Bioconductor
|
| |
|
| Submission date |
Dec 04, 2015 |
| Last update date |
Dec 04, 2015 |
| Contact name |
Giovanni Coppola |
| E-mail(s) |
[email protected]
|
| Phone |
310-794-4172
|
| Organization name |
UCLA
|
| Department |
Psychiatry and Neurology
|
| Lab |
Neurogenetics
|
| Street address |
1524 Gonda, 695 Charles Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL6100 |
| Series (1) |
| GSE35363 |
Lipocalin 2 is present in the EAE brain and is modulated by natalizumab |
|
