|
| Status |
Public on Jul 01, 2017 |
| Title |
miR-200c m6A modified |
| Sample type |
RNA |
| |
|
| Source name |
miR-200c m6A modified oligonucleotide transfected cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RKO
cell type: colorectal cancer cell line
genotype: DICER exon 5-disrupted
|
| Treatment protocol |
Double stranded RNA oligonucleotides are transfected to cells with lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocols. RNA was extracted 48hours after transfection.
|
| Growth protocol |
DICER exon 5-disrupted RKO cells are were maintained in Roswell Park Memorial Institute (RPMI) 1640 Medium supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) and grown at 37°C in a humidified incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells by TRIzol (Invitrogen) according to manufacturer’s instructions.
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
miR-200c m6A modified oligonucleotide transfected cells
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Dec 11, 2015 |
| Last update date |
Jul 01, 2017 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE75927 |
Influence of RNA modification on microRNAs |
|
