|
| Status |
Public on Oct 01, 2019 |
| Title |
DLD1 Glc+Gln |
| Sample type |
RNA |
| |
|
| Source name |
DLD1 under glucose- and glutamine-containing conditions
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: colorectal cancer
cell line: DLD1
condition: glucose- and glutamine-containing conditions
|
| Growth protocol |
DLD1 and HT29 cells were cultured in complete medium; the medium was replaced the following day with glucose(−) and glutamine(−) medium supplemented with 10% dialysed fetal bovine serum. Glucose (10 mM) or glutamine (2 mM) was added, respectively. After 24 h incubation, cellular RNA was extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was purified from cultured cells using the RNeasy mini kit (Qiagen, Valencia, CA, USA) following the manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Dec 21, 2015 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE76236 |
The metabolism of colorectal cancer adapting to nutritional stress |
|
