|
| Status |
Public on Mar 11, 2008 |
| Title |
chicken DNA, biological rep2 |
| Sample type |
genomic |
| |
|
| Source name |
liver
|
| Organism |
Gallus gallus |
| Characteristics |
female
|
| Treatment protocol |
No treatment.
|
| Growth protocol |
Eight week-old female quail and chicken (WL-G) kept under long day condition (18 h light: 6h dark) were used.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using DNeasy tissue kit (QIAGEN) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated DNA were labelled by BioPrime DNA labelling system (Invitrogen) according to manufacturer's protocol from 0.3 ug genomic DNA.
|
| |
|
| Hybridization protocol |
Ten ug of labelled DNA were hybridized for 16 h at 45 C on Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
data of genomic DNA hybridization
|
| Data processing |
The data were analyzed with GeneChip Operating Software version 1.4 (GCOS 1.4) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 05, 2007 |
| Last update date |
Mar 11, 2008 |
| Contact name |
Takashi Yoshimura |
| E-mail(s) |
[email protected]
|
| Phone |
+81-52-789-4056
|
| Fax |
+81-52-789-4056
|
| Organization name |
Nagoya University
|
| Department |
Graduate School of Bioagricultural Sciences
|
| Lab |
Lab. of Animal Functional Genomics
|
| Street address |
Furo-cho, Chikusa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
464-8601 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE8016 |
Comparative genomic hybridization between chicken and Japanese quail |
|
