|
| Status |
Public on Mar 11, 2008 |
| Title |
quail, short day condition at ZT22, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mediobasal hypothalamus, short day condition (6h light:18h dark), 2week
|
| Organism |
Coturnix japonica |
| Characteristics |
male
|
| Treatment protocol |
At the age of 8 weeks old, birds were devided into two groups. One group was continuously kept under short day condition (6h light:18h dark) for 2 weeks. And the other group was transferred to long day condition (20h light: 4h dark) for 2 weeks.
|
| Growth protocol |
Eight week-old male quail kept under short day condition (6h light:18h dark) for 4 weeks were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 h at 45 C on GeneChip Checken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
gene expression data of MBH at short day condition ZT22
|
| Data processing |
The data were analyzed with GeneChip Operating Software version 1.2 (GCOS 1.2) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 05, 2007 |
| Last update date |
Mar 11, 2008 |
| Contact name |
Takashi Yoshimura |
| E-mail(s) |
[email protected]
|
| Phone |
+81-52-789-4056
|
| Fax |
+81-52-789-4056
|
| Organization name |
Nagoya University
|
| Department |
Graduate School of Bioagricultural Sciences
|
| Lab |
Lab. of Animal Functional Genomics
|
| Street address |
Furo-cho, Chikusa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
464-8601 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE8018 |
Gene expression in the quail MBH under short and long day conditions. |
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