|
| Status |
Public on Jan 25, 2017 |
| Title |
Neutrophils from sJIA sample 51 before treatment |
| Sample type |
RNA |
| |
|
| Source name |
Neutrophils from sJIA sample 51 before treatment
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Neutrophils
disease state: sJIA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were prepared using the GeneChip eukaryotic small sample target labeling assay, version II, with 100 ng of RNA and an Affymetrix GeneChip IVT labeling kit (Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix U133 Plus 2.0 arrays.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from Neutrophils (sJIA sample 51) before treatment
|
| Data processing |
Processing of Affymetrix data was performed in GeneSpring GX11.0 (Agilent) using GCRMA (RMA; Robust Multi-array Analysis that accounts for the probes GC content) method for normalizing and summarizing probe-level intensity measurements. The CHD file was generated in Affymetrix Expression console build 1.4.1.46 using RMA.
|
| |
|
| Submission date |
Jan 04, 2016 |
| Last update date |
Jan 25, 2017 |
| Contact name |
Ebun Omoyinmi |
| E-mail(s) |
[email protected]
|
| Phone |
020 79052392
|
| Organization name |
UCL, Institute of Child Health
|
| Department |
Infection, Inflammation and Rheumatology
|
| Street address |
30 Guilford Street
|
| City |
London |
| ZIP/Postal code |
WC1N 1EH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE76492 |
Mitochondrial and oxidative stress genes are differentially expressed in neutrophils of sJIA patients treated with tocilizumab: a pilot microarray study |
|
