|
| Status |
Public on Jun 07, 2016 |
| Title |
MDA_tRNA-Glu-OE_0h_r2 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB-231
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
overexpression: tRNA-Glu
time point: 0
|
| Treatment protocol |
Cells were treated with a-amanitin (10ug/mL) for 0 or 9 hours prior to RNA extraction.
|
| Growth protocol |
Cells were grown in DMEM-based media supplemented with 10% FBS, 1% L-glutamine, 1% Sodium Pyruvate, and 1% Pen/Strep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and on-column Dnase treated using Norgen Biotek total RNA extraction kit (per manufacturer instructions).
|
| Label |
biotin
|
| Label protocol |
Samples were biotin-labeled using the TargetAmp-Nano Labeling Kit for Ilumina Expression BeadChip
|
| |
|
| Hybridization protocol |
Per manufacturer's instruction
|
| Scan protocol |
Per manufacturer's instruction
|
| Description |
replicate 2
|
| Data processing |
Data were quantile-normalized and processed using the Lumi package. Non-normalized values indicate the average probe hybrydization signal. Further processed data representing logFC of samples relative to control are linked to the Series record.
|
| |
|
| Submission date |
Jan 28, 2016 |
| Last update date |
Jun 07, 2016 |
| Contact name |
Hani Goodarzi |
| Organization name |
UCSF
|
| Department |
Biochemistry and Biophysics
|
| Street address |
600 16th St, GH S312D
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE77349 |
Impact of tRNA-Glu(UUC) and tRNA-Arg(CCG) on mRNA stability |
| GSE77401 |
Modulated expression of specific tRNA species drives cancer progression |
|
