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Sample GSM2052424 Query DataSets for GSM2052424
Status Public on Apr 06, 2016
Title intestine tissue 2
Sample type RNA
 
Source name intestine tissue
Organism Mus musculus
Characteristics strain: C57BL/6
tissue/cell type: intestine tissue
Treatment protocol Differentiated gallbladder organoids were obtained by culturing the organoids in media lacking R-spondin 1, noggin and nicotinamide for two weeks.
Growth protocol Organoids were cultured in Matrigel with tissue culture media based on AdDMEM/F12 (Life Technologies) and supplemented with B27, N2 (Life Technologies) and 1.25 uM N-Acetylcysteine (Sigma-Aldrich). The following growth factors were also added: 50 ng/mL murine recombinant EGF (Life Technologies), 50 ng/mL murine recombinant FGF10 (R&D system), 10 mM nicotinamide (Sigma-Aldrich), 50 ng/mL murine recombinant HGF (Peprotech) and R-spondin 1 and noggin home-made conditioned media (prepared as in Sato et al., Nature 459: 262-265, 2009).
Extracted molecule total RNA
Extraction protocol RNA was extracted from liver, gallbladder or small intestine tissues, and liver, gallbladder or small intestine organoids using the RNeasy Mini RNA Extraction Kit (Qiagen).
Label biotin
Label protocol 5.5 ug of ds cDNA were fragmented and labeled using the GeneChip WT DNA terminal labeling kit.
 
Hybridization protocol Biotinylated cRNA was fragmented and hybridized to GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix) following the standard protocol of the manufacturer.
Scan protocol Standard Affymetrix.
Description IT2
Data processing RMA Probes to Import: Interrogating Probes, Probe filtering: skip,Algorithm: RMA,Background Correction: RMA Background Correction,Normalization: Quantile Normalization,Log Probes using Base: 2,Probeset Summarization: Median Polish
 
Submission date Feb 02, 2016
Last update date Apr 06, 2016
Contact name Thanos Halazonetis
E-mail(s) [email protected]
Organization name University of Geneva
Department IDepartment of Molecular Biology
Lab Thanos Halazonetis
Street address Sciences III, 30, quai Ernest-Ansermet
City Geneva
State/province GE
ZIP/Postal code 1211
Country Switzerland
 
Platform ID GPL6246
Series (1)
GSE77461 R-spondin 1 and noggin facilitate expansion of resident stem cells from non-damaged gallbladders.

Data table header descriptions
ID_REF
VALUE Log2 normalized

Data table
ID_REF VALUE
10338001 12.1301
10338002 7.96638
10338003 10.7345
10338004 9.09579
10338005 3.11114
10338006 3.56088
10338007 4.2568
10338008 5.36131
10338009 10.1458
10338010 3.18982
10338011 7.43212
10338012 3.39881
10338013 2.96301
10338014 3.06331
10338015 2.97108
10338016 9.26839
10338017 12.9293
10338018 8.50562
10338019 6.8314
10338020 9.90536

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM2052424_Lugli_0113_I2_MoGene-1_0-st-v1_.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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