|
| Status |
Public on Aug 09, 2016 |
| Title |
Melanosomes WM3314_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
WM3314 melanosomes
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: WM3314
cell component: melanosomes
|
| Treatment protocol |
Melanosomes of WM3682 and WM3314 cells were treated with RNase for 10 min at 37 °C, followed by the addition of RNase inhibitor.
|
| Growth protocol |
MNT-1, WM3682 and WM3314 cells were cultured in DMEM medium supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% L-glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA of WM3682 and WM3314 cells and melanosomes was extracted with Trizol reagent. RNA from MNT-1 exosomes and secreted melanosomes was isolated using the miRNeasy Mini Kit from Qiagen. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled according to the Agilent miRNA Complete Labeling and Hyb kit protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed in a SureHyb chamber at 55°C for 20h.
|
| Scan protocol |
Microarray scanning was performed using the Agilent Scanner G2505C.
|
| Description |
RNA from melanosomes of WM3314 cells
MEL 3314
|
| Data processing |
The data were quantile normalized and fold-changes were calculated using R/Bioconductor scripts.
|
| |
|
| Submission date |
Mar 20, 2016 |
| Last update date |
Aug 09, 2016 |
| Contact name |
Carmit Levy |
| E-mail(s) |
[email protected]
|
| Organization name |
Tel Aviv University
|
| Department |
Human Genetics and Biochemistry
|
| Lab |
Sackler School of Medicine
|
| Street address |
Ramat Aviv
|
| City |
Tel Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL21576 |
| Series (2) |
| GSE72620 |
Melanoma miRNA trafficking triggers the tumor primary niche formation |
| GSE79411 |
Analysis of melanosomal, exosomal and cellular miRNAs of melanoma cells |
|
