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Status |
Public on Jul 01, 2016 |
Title |
US-1419278_MK0646+MK8669_R2 |
Sample type |
SRA |
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Source name |
Xenograft tumor tissue
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Organism |
Homo sapiens |
Characteristics |
cell line: MCF-7/AC-1 time: Post treatment (Day 28) treatment: MK-0646+MK-8669
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Extracted molecule |
total RNA |
Extraction protocol |
Xenograft tumors were rapidly excised, weighed and preserved in RNA Later (Invitrogen Life Technologies, Carlsbad, CA, USA). Total RNA was isolated using Trizol reagents (Cat#15596-026, Invitrogen, Carlsbad, CA). RNA integrity was confirmed using the Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
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Data processing |
Next-generation sequencing was performed using the Illumina HiSeq 2000 platform. RNA-seq data was analyzed with Partek Flow 3.0. Reads were mapped to the human genome (Ensembl GRCh37) and quantified using the STAR aligner. Aligned reads to genes using Partek E/M. Differentially expressed genes determined using gene-specific analysis (GSA). Genome_build: GRCh37 Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each sample
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Submission date |
Mar 22, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Marc A Becker |
E-mail(s) |
[email protected]
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Phone |
507-284-2511
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Organization name |
Mayo Clinic
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Department |
Medical Oncology
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Street address |
200 First St SW
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City |
Rochester |
State/province |
MN |
ZIP/Postal code |
55905 |
Country |
USA |
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Platform ID |
GPL11154 |
Series (1) |
GSE79492 |
Ridaforolimus (MK-8669) synergizes with Dalotuzumab (MK-0646) in hormone-sensitive breast cancer |
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Relations |
BioSample |
SAMN04572553 |
SRA |
SRX1653252 |
Supplementary file |
Size |
Download |
File type/resource |
GSM2095971_US-1419278_FPKM.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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