|
| Status |
Public on Jul 21, 2007 |
| Title |
RNA from T98G cells in S phase of the cell cycle |
| Sample type |
RNA |
| |
|
| Source name |
T98G cells
|
| Organism |
Homo sapiens |
| Characteristics |
human glioblastoma cell line
|
| Growth protocol |
RNA was purified from T98G cells that were either cycling, G0 arrested, or released into S phase for 10 hr and 16 hr. For each experimental condition, 15 μg of RNA was processed and hybridized against Affymetrix GeneChip Human Genome U133 Plus 2.0 Array.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method of RNA isolation and purification by Rneasy mini kit vy Qiagen
|
| Label |
biotin
|
| Label protocol |
RNA from each sample is converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA. The cRNA is hybridized to a microarray chip. The bound cRNA is stained with fluorescent streptavidin.
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Description |
RNA from T98G cells in S phase of the cell cycle
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Jul 20, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Subhashini Sadasivam |
| E-mail(s) |
[email protected]
|
| Organization name |
Dana Farber Cancer Insitute
|
| Department |
Medical Oncology
|
| Lab |
Laboratory of James DeCaprio, Mayer 444
|
| Street address |
450 Brookline Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8537 |
Gene expression profilie during cell cycle in T98G cells |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
