|
| Status |
Public on Jan 20, 2017 |
| Title |
HDF H2O2_1d |
| Sample type |
RNA |
| |
|
| Source name |
HDF
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human diploid fibroblast (HDF)
treated with: hydrogen peroxide for 1 day
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent(Invitrogen Life Technologies, Carlsbad, USA), purified using RNeasy columns (Qiagen, Valencia, USA) according to the manufacturers’ protocol. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 0.55 ug total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin,TX).
|
| |
|
| Hybridization protocol |
Following fragmentation, 0.75 ug of cRNA were hybridized to the Illumina HumanHT-12 Expression Beadchip according to the protocols provided by the manufacturer
|
| Scan protocol |
Arrays were scanned using the Illumina Bead Array Reader Confocal Scanner
|
| Description |
HDF H2O2_1d.AVG_Signal
|
| Data processing |
Array data export processing and analysis was performed using Illumina GenomeStudio v2009.2
|
| |
|
| Submission date |
Apr 15, 2016 |
| Last update date |
Jan 20, 2017 |
| Contact name |
Hyun Goo Woo |
| E-mail(s) |
[email protected]
|
| Organization name |
Ajou University School of Medicine
|
| Street address |
164 Worldcup-ro Yeongtong-gu
|
| City |
Suwon |
| ZIP/Postal code |
16499 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE80322 |
UHRF1 is an upstream regulator of DNMT1-mediated cellular senescence |
|
