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Sample GSM215693 Query DataSets for GSM215693
Status Public on Dec 18, 2008
Title Rat17 LUSH 3D rep2
Sample type RNA
 
Source name lung tissue
Organism Rattus norvegicus
Characteristics exposed to sustained hypoxia: 3D
Treatment protocol Rats subject to the intermittent hypoxia (IH) treatment were exposed to 90 second cycles of 10% inspired oxygen followed by 90 seconds of 21% inspired oxygen for 12 hours of simulated daylight when rats sleep; for the remaining 12 hours during the night, the rats were exposed to 21% inspired oxygen. Rats subject to the sustained hypoxia (SH) treatment were exposed to 10% inspired oxygen at all times. Time sacrifices of these rats exposed to IH and SH respectively were performed on days 1, 3, 7, 14, and 30.
Growth protocol Adult inbred Sprague-Dawley male rats (weights: 250-275g) were placed in chambers in which oxygen and carbon dioxide concentrations were carefully monitored and controlled.
Extracted molecule total RNA
Extraction protocol To prepare RNAs for microarray experiments and quantitative RT-PCRs, 1 ml per 50 mg of frozen lung tissues were homogenized in ice cold Trizol, and phase separated with Chlorophorm (0.2ml per 1ml Trisol reagent). The RNAs were precipitated from the aqueous phase using Isopropanol (0.5ml per 1ml Trisol). The RNA pallet then was washed with 75%Ethanol. Next the extracted RNAs were purified further using the RNeasy Mini Cleanup protocol (Qiagen 74104) according to the manufacturer’s recommendations. The quantities of RNAs were determined by OD measurements at 260nm, and their qualities were assessed by Agilent Bioanalyzer and by running the RNAs on formamide formaldehyde denaturing gels
Label Cy5
Label protocol Briefly, the RNAs extracted from whole lung tissue of each rat (mixed with spiked-in bacterial RNAs served as positive controls) were reverse transcribed into double stranded cDNAs; then the cDNAs were amplified into cRNAs by in vitro transcription. The cRNAs were labeled with a red (Cy5) fluorescent dye and hybridized to a CodeLink Bioarray which is presynthesized with probes.
 
Hybridization protocol According to standard CodeLink protocols; see details at http://www1.gelifesciences.com/APTRIX/upp00919.nsf/Content/WD%3ACodeLink+Gene+E%28281090525-B500%29?OpenDocument&hometitle=WebDocs
Scan protocol Microarray slides were scanned using a GenePix Array Scanner. For details, see the protocol at http://www1.gelifesciences.com/APTRIX/upp00919.nsf/Content/WD%3ACodeLink+Gene+E%28281090525-B500%29?OpenDocument&hometitle=WebDocs
Description LUSH3D14V
Data processing The raw intensity values were normalized by the CyclicLoess normalization method as described in Wu W, et. al, BMC Bioinformatics. 2005 Dec 28;6:309. The data was also preprocessed and postprocessed using the protocol described in the same paper and the submitted paper.
 
Submission date Aug 06, 2007
Last update date Aug 14, 2011
Contact name Wei Wu
E-mail(s) [email protected]
Phone 412-647-3156
Fax 412-647-7875
Organization name University of Pittsburgh
Department Medicine/PACCM
Street address MUH 628 NW, 3459 Fifth Ave
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL2890
Series (1)
GSE8705 Temporal effects of intermittent- and sustained hypoxia on gene expression patterns in rat lungs

Data table header descriptions
ID_REF
VALUE Normalized Intensity values by CyclicLoess; data was log2-transformed, and processed as described in a submitted manuscript.

Data table
ID_REF VALUE
109 9.03175
110 7.77575
111 9.34375
112 6.89875
113 10.03875
114 7.87775
115 8.82775
116 8.11075
117 6.85475
118 10.83875
119 4.73575
120 11.37875
121 7.48675
122 5.83375
123 9.00975
124 5.79975
125 10.29875
126 10.67875
127 8.16475
128 7.49175

Total number of rows: 10014

Table truncated, full table size 131 Kbytes.




Supplementary file Size Download File type/resource
GSM215693.txt.gz 927.8 Kb (ftp)(http) TXT
Processed data included within Sample table

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