|
| Status |
Public on Nov 15, 2016 |
| Title |
PB_CD8aa_rep3 |
| Sample type |
SRA |
| |
|
| Source name |
CD8αα T cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD8aa
cell derivation: Peripheral blood
stimulation method: CD3/28 beads with IL-7 and IL-15
days after stimulation: 7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using an RNeasy Plus Mini Kit (QIAGEN)
Libraries for RNA-sequence were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Basecalls performed using CASAVA
Sequence alignment performed using our in-house pipelines (Genomon Fusion).
Fragments per kilobase of exon per million fragments mapped (FPKM) was calculated for Refseq genes and then log-transformed.
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
|
| |
|
| Submission date |
May 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Takuya Maeda |
| E-mail(s) |
[email protected]
|
| Phone |
81-75-751-3818
|
| Organization name |
Institute for Frontier Medical Science, Kyoto University
|
| Department |
Department of Immunology
|
| Street address |
53 Kawahara-cho Shogoin Sakyo-ku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
| GSE81975 |
Gene expression profile of regenerated CD8αα T cells and CD8αβ T cells from LMP2 T-iPSCs |
|
| Relations |
| BioSample |
SAMN05180915 |
| SRA |
SRX1802840 |
