|
| Status |
Public on Aug 01, 2016 |
| Title |
MEF-N4, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblasts expressing human HMGN4 (MEF-N4)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: embryonic fibroblasts
cell line: SV40-immortalized (ATCC, CRL-2907)
vector: human HMGN4 (MEF-N4)
|
| Treatment protocol |
Cells were harvested by trypsinization
|
| Growth protocol |
SV40-immortalized mouse embryonic fibroblast (ATCC) cell lines were maintained in Dulbecco's modified Eagle's medium, supplemented with 10% fetal calf serum, at 37˚C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
|
| |
|
| Hybridization protocol |
Biotininylated probes were hybridized to Affymetrix Mouse Gene 1.0 ST arrays using Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from mouse embryonic fibroblasts
|
| Data processing |
Data were analyzed by BRB Array Tools, GeneSpring GX 12.6 (Agilent Technologies) and Partek Genomics Suite 6.5 software
|
| |
|
| Submission date |
Jun 13, 2016 |
| Last update date |
Aug 01, 2016 |
| Contact name |
Yuri Postnikov |
| E-mail(s) |
[email protected]
|
| Phone |
301-496-2885
|
| Organization name |
NIH
|
| Street address |
37 Convent Dr
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE83313 |
Expression data from human HMGN4- and HMGN2-expressing mouse embryionic fibroblasts |
|
