|
| Status |
Public on Nov 22, 2017 |
| Title |
D_MG_223_CD14 |
| Sample type |
RNA |
| |
|
| Source name |
Monocyte RNA from female MG discordant twins pair22
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: CD14 purified from PBMC
age: 21 years
clincal phenotype: MG
twin status: Twin pair 22
arrayrun: II
gender: Female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMCs were purified from 60 ml blood by Ficoll density gradient centrifugation (U16 NovaMed). The CD14 subset was isolated using EasySep™ Human Monocyte Enrichment Kit (#19059 Stemcell). Recovery was 65-80% with a purity > 95% for all cell-subsets. From each sample both RNA and DNA was extracted using AllPrep kit (#80204 QIAGENE) according to manufacturer's protocol. DNA concentration was measured by picogreen and stored at 4°C until use. RNA quantity and quality was measured by NanoDrop and Agilent BioAnalyzer respectively and stored at -80°C until use.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the TargetAmp™- Nano Labeling Kit for Illumina® Expression BeadChip®
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Data was log2 transformed data and filtered for non-expressed genes (detection p-value<0.01), and for low variance transcripts across samples (variance <5%). Data was normzlaized using loess normaliztion and analyzed using one-way ANOVA using JMP Genomics V7 software (SAS Institute Inc, Cary, NC)
|
| |
|
| Submission date |
Aug 10, 2016 |
| Last update date |
Nov 22, 2017 |
| Contact name |
Sonia Berrih-Aknin |
| E-mail(s) |
[email protected]
|
| Organization name |
UPMC - Inserm UMRS 974, CNRS FRE3617
|
| Department |
Sorbonne Universités
|
| Lab |
Center of Research in Myology
|
| Street address |
105 Boulevard de l’Hôpital
|
| City |
Paris |
| State/province |
State... |
| ZIP/Postal code |
75651 |
| Country |
France |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE85452 |
Methylation and expression profiles of monocytes in monozygotic twins: role in Myasthenia Gravis [expression profiling] |
| GSE85649 |
Methylation and expression profiles of monocytes in monozygotic twins: role in Myasthenia Gravis |
|
