|
| Status |
Public on Mar 28, 2017 |
| Title |
METS (metastasis-derived DLD1 derivative) colorectal cancer cell line, replicat 2 |
| Sample type |
RNA |
| |
|
| Source name |
colorectal cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
parental cell type: colorectal cancer cell line DLD1
cell characteristics: epithelial, derived from xenograft lung metastasis
|
| Treatment protocol |
no treatment
|
| Growth protocol |
Colorectal cancer cell line DLD1 and its derivatives were maintained in McCoy`s 5A Medium (Invitrogen) containing 10% fetal bovine serum (FBS, Invitrogen), 100 units/ml penicillin and 0.1 mg/ml streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from cell lines was isolated using the miRNeasy Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Standard Agilent Protocol for Cy3 labeling
|
| |
|
| Hybridization protocol |
Standard Agilent Protocol
|
| Scan protocol |
Standard Agilent Protocol
|
| Description |
METS (metastasis-derived DLD1 derivative) colorectal cancer cell line
|
| Data processing |
Quantile normalization was performed using the preprocessCore package with R version 3.2.0
|
| |
|
| Submission date |
Aug 16, 2016 |
| Last update date |
Mar 29, 2017 |
| Contact name |
Matjaz Rokavec |
| E-mail(s) |
[email protected]
|
| Organization name |
Ludwig-Maximilians-University Munich
|
| Department |
Institute of Pathology
|
| Lab |
Hermeking
|
| Street address |
Thalkirchner Str. 36
|
| City |
Munich |
| ZIP/Postal code |
80337 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21576 |
| Series (2) |
| GSE85687 |
microRNA profiling of four DLD1 colorectal cancer cell derivatives that recapitulate EMT/MET transitions during metastasis. |
| GSE85688 |
Cellular model of colon cancer progression reveals signatures of mRNAs, miRNA, lncRNAs and epigenetic modifications associated with metastasis. |
|
