|
| Status |
Public on Aug 23, 2016 |
| Title |
HCT116 Dicer intermediate cells |
| Sample type |
RNA |
| |
|
| Source name |
HCT116 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Colon carcinoma
|
| Treatment protocol |
For Hct tet100 cells, Dicer gene disrupted HCT116 cells were transduced with tet-on Dicer gene and cultured with 100 pg/ml tetracycline for more than one week.
|
| Growth protocol |
DMEM with 10% FBS with or without tetracycline
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard Trizol Reagent for the RNA extraction.
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
Intermediate levels of Dicer protein expression
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Aug 22, 2016 |
| Last update date |
Aug 23, 2016 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE85909 |
Comprehensive gene expression changes by the expression levels of Dicer protein |
|
