|
| Status |
Public on Nov 01, 2007 |
| Title |
H9 embryonic stem cells (endothelial study) |
| Sample type |
RNA |
| |
|
| Source name |
H9 undifferentiated embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
derived from H9
|
| Treatment protocol |
see supplementary information Lu et.al, Nature Methods, 2007 Jun;4(6):501-9.
|
| Growth protocol |
see supplementary information Lu et.al, Nature Methods, 2007 Jun;4(6):501-9.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Qiagen RNAeasy kiit and was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr.
|
| Scan protocol |
Once the probe array has been hybridized, washed, and stained, it is scanned.
|
| Description |
H9-ES
|
| Data processing |
The data were analyzed with Robust Multi-Chip Analysis (RMA).
|
| |
|
| Submission date |
Sep 18, 2007 |
| Last update date |
Jan 14, 2015 |
| Contact name |
Jennifer A Hipp |
| E-mail(s) |
[email protected]
|
| Organization name |
Wake Forest School of Medicine
|
| Department |
Institute for Regenerative Medicine
|
| Lab |
Atala
|
| Street address |
Medical Center Blvd
|
| City |
Winston-Salem |
| State/province |
NC |
| ZIP/Postal code |
27157 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE9089 |
Reanalysis of GSE8884 Samples with Endothelial Samples from GSE3998. |
| GSE9196 |
Comparison of ES, EB, and Blast cells to breast epithelial, leuckocytes, endothelial and stromal cells |
|
| Relations |
| Reanalysis of |
GSM225045 |
| Reanalyzed by |
GSE64985 |
