|
| Status |
Public on Sep 27, 2007 |
| Title |
Knockout saline, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Nucleus Accumbens from knockout saline treated mice
|
| Organism |
Mus musculus |
| Characteristics |
bilateral, 1mm-thick, 15gauge punches, pooled together from four mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TriZol and purified with RNAeasy Micro kit.
|
| Label |
biotin
|
| Label protocol |
linear amplification kit Ambion #IL1791. Input amount: 200ng of total RNA, IVT 2hrs.
|
| |
|
| Hybridization protocol |
16hrs at 55degress C using Illumina reagents
|
| Scan protocol |
Standard scan factor using Illumina Scanner
|
| Description |
Nucleus Accumbends, Male 8-12 week old HDAC5 WT or KO mice treated with chronic (7 daily injections) saline or cocaine (20mg/kg).
|
| Data processing |
The Raw data was extracted using MS5, and median-normalized.
|
| |
|
| Submission date |
Sep 21, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Will Renthal |
| Organization name |
Brigham and Women's Hospital
|
| Street address |
60 Fenwood Rd.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL4234 |
| Series (1) |
| GSE9134 |
Genome-wide gene expression in HDAC5 wildtype and knockout mice treated with chronic cocaine or saline |
|
