|
| Status |
Public on Nov 10, 2007 |
| Title |
Allergic_saline_pt3 |
| Sample type |
RNA |
| |
|
| Source name |
epithelial cells cultured from biobsy, saline exposed 24h
|
| Organism |
Homo sapiens |
| Characteristics |
monotypic nouse dust mite allergic
|
| Treatment protocol |
cells were preincubated with HBSS 8 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
|
| Growth protocol |
Cells were isolated from biobsy by digesting biopsy with collagenase, cells were cultured in BEGM culture medium for app. 2 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from cells after exposure to saline or HDM
|
| Data processing |
The data were analyzed with Affymetrix GeneChip® Operating Software using Affymetrix default analysis settings. Data were normalized using the robust multi-array average (RMA) algorithm
|
| |
|
| Submission date |
Sep 24, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Aram Ben Vroling |
| E-mail(s) |
[email protected]
|
| Organization name |
AMC
|
| Department |
ENT
|
| Lab |
ENT
|
| Street address |
meibergdreef 9
|
| City |
Amsterdam |
| ZIP/Postal code |
1100DD |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9150 |
Primary nasal epithelium exposed to house dust mite extract shows activated expression in allergics |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
