|
| Status |
Public on Apr 01, 2009 |
| Title |
NKX2-8_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Transfectant cells overexpressing NKX2-8
|
| Organism |
Homo sapiens |
| Characteristics |
Blasticidin-selected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from stable transfectant cells over-expressing individual transcription factors was extracted using the QiaShredder and Qiagen RNeasy Mini kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
The resulting biotin-labeled RNA samples were then hybridized with Affymetrix U133 Plus 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from cell-lines transfected by NKX2-8.
|
| Data processing |
The data were analyzed using Stratagene's ArrayAssist Enterprise using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Oct 02, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Chaitanya R Acharya |
| E-mail(s) |
[email protected]
|
| Phone |
919-681-8350
|
| Organization name |
Duke University
|
| Department |
Dept. of Surgery
|
| Lab |
Lyerly Lab
|
| Street address |
203 Research Drive,Medical Sciences Research Building, Suite 433
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9212 |
Overexpression of lung developmental transcription factors TTF-1, NKX2-8 and PAX9 |
|
