|
| Status |
Public on Sep 01, 2018 |
| Title |
PCOS 153A |
| Sample type |
RNA |
| |
|
| Source name |
PCOS, ovary sample
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
disease state: PCOS
tissue: ovary
|
| Treatment protocol |
There was no treatment on the tissues.
|
| Growth protocol |
All tissue samples were snap frozen in liquid nitrogen immediately after collection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from Ovary biopsies using TRIZOL reagent (Invitrogen, Breda, The Netherlands) as per manufacturer's instructions. After Trizol purification, RNA was repurified using the Rneasy Mini Kit (Qiagen, Venlo., The Netherlands) according to manufacturer's instructions. Total RNA was quantified by OD260/OD280 measurement and with RiboGreen RNA Quantitation Kit (Moleular Probes, Leiden, The Netherlands). RNA quality was assessed on a 6000 nanochip using Agilent 2100 bioanalyzer (Agilent Technologys, Waldbronn, Germany).
|
| Label |
biotin
|
| Label protocol |
cRNA was biotin-labeled.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Affymetrix protocols.
|
| Scan protocol |
Chips were scanned on an Affymetrix Scanner according to the manufacturers protocol.
|
| Description |
Gene expression data from ovary biopt material
|
| Data processing |
The data were analyzed with Partek Genomics Suite version 6.6 using GCRMA with default settings and quantile normalization method. The data is log2 transformed.
|
| |
|
| Submission date |
Sep 28, 2016 |
| Last update date |
Sep 01, 2018 |
| Contact name |
Arne IJpma |
| Organization name |
Erasmus MC
|
| Department |
Bioinformatics
|
| Street address |
Dr. Molewaterplein 50
|
| City |
Rotterdam |
| ZIP/Postal code |
3000 CA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE87435 |
Microarray expression data derived from ovaries of women with Polycystic Ovary Syndrome and Female to Male Transexual individuals after treatment with Testosterone |
|
