|
| Status |
Public on May 22, 2017 |
| Title |
SLX-9772.N709_N517 |
| Sample type |
SRA |
| |
|
| Source name |
Hematopoietic Stem Cell
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole bone marrow
cell type: Hematopoietic Stem Cell
age: 18 Months (Old)
genotype: JAK2 V617F Homozygous
|
| Treatment protocol |
not applicable
|
| Growth protocol |
not applicable
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
hematopoietic stem cells were isolated from whole bone marrow using ESLAM markers foir FACS sorting
The libraries were constructed according to Picelli et al 2014 using Nextera Illumina Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Old HSC JAK2 HOM
|
| Data processing |
Illumina basecalling
Reads mapped to Mouse GRC38-77 reference genome from ENSEMBL plus ERCC92 spike-in controls with tophat 2.0.13 and bowtie 2.2.3
Gene expression count matrix was calculated with htseq
Genome_build: GRC38 release 77
Supplementary_files_format_and_content: Tabular separated raw counts. First column is the genes, second column is the counts.
|
| |
|
| Submission date |
Oct 05, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Anthony Green |
| Organization name |
University of Cambridge
|
| Department |
Department of heamatology
|
| Lab |
Green's Lab
|
| Street address |
Hills Road
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE87631 |
Single Cell RNA-Sequencing using smart-seq2 of young and old murine hematopoietic stem cells |
|
| Relations |
| BioSample |
SAMN05863671 |
| SRA |
SRX2211194 |
