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Sample GSM2336234 Query DataSets for GSM2336234
Status Public on May 22, 2017
Title SLX-9774.N704_N504
Sample type SRA
 
Source name Hematopoietic Stem Cell
Organism Mus musculus
Characteristics tissue: whole bone marrow
cell type: Hematopoietic Stem Cell
age: 4 Months (young)
genotype: JAK2 V617F Homozygous
Treatment protocol not applicable
Growth protocol not applicable
Extracted molecule polyA RNA
Extraction protocol hematopoietic stem cells were isolated from whole bone marrow using ESLAM markers foir FACS sorting
The libraries were constructed according to Picelli et al 2014 using Nextera Illumina Kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description Young HSC JAK2 HOM
Data processing Illumina basecalling
Reads mapped to Mouse GRC38-77 reference genome from ENSEMBL plus ERCC92 spike-in controls with tophat 2.0.13 and bowtie 2.2.3
Gene expression count matrix was calculated with htseq
Genome_build: GRC38 release 77
Supplementary_files_format_and_content: Tabular separated raw counts. First column is the genes, second column is the counts.
 
Submission date Oct 05, 2016
Last update date May 15, 2019
Contact name Anthony Green
Organization name University of Cambridge
Department Department of heamatology
Lab Green's Lab
Street address Hills Road
City Cambridge
State/province Cambridgeshire
ZIP/Postal code CB2 0XY
Country United Kingdom
 
Platform ID GPL17021
Series (1)
GSE87631 Single Cell RNA-Sequencing using smart-seq2 of young and old murine hematopoietic stem cells
Relations
BioSample SAMN05863119
SRA SRX2211360

Supplementary file Size Download File type/resource
GSM2336234_SLX-9774.N704_N504.txt.gz 115.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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