strain: Wistar tissue: liver infection status: infected with Clonorchis sinensis gender: male
Treatment protocol
The animals were sacrificed at 3 weeks post-infection and the livers were harvested and preserved in TRIzol reagent (Shanghai Invitrogen Biotechnology Co., Ltd., Shanghai, China) at -80°C, according to the manufacturer’s protocol, until RNA extraction.
Growth protocol
Wistar rats (5–6 weeks old, male) were housed in an air-conditioned room at 24°C under a 12-h dark/light cycle with free access to standard laboratory food and water. The rats were individually infected orally with 50 metacercariae. Parasitic infection was assessed by detection of parasite eggs in stool samples and pathological findings. Mock-infected control rats were similarly administered with 50 μl of sterile normal solution. All animal care and experimental procedures were conducted in accordance with the guidelines for animal use in toxicology.
Extracted molecule
total RNA
Extraction protocol
RNA was extracted by mirVanaTM RNA Isolation Kit (Applied Biosystem p/n AM1556 ) following the manufacturer's instructions.
Label
biotin
Label protocol
Total RNA were amplified, labeled and purified by using FlashTag Biotin HSR RNA Labeling Kit (Cat#901911, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
Hybridization protocol
Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions
Scan protocol
Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software 3.1 (Affymetrix, Santa Clara, CA, US) with default settings.
Description
liver gene expression of rats at 3 weeks post-infection with clornorchis sinensis
Data processing
Raw data were normalized by RMA algorithm, Expression Console(version1.3.1, Affymetrix).
