|
| Status |
Public on Nov 27, 2007 |
| Title |
Day 22-35 Chronic infection GP33 specific-2_MG-U74B |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Spleen CD8 T cells
|
| Organism |
Mus musculus |
| Characteristics |
C57Bl/6
Day 22-35 Chronic LCMV infection
|
| Treatment protocol |
Virus specific CD8 T cells were sorted by flow cytometry and purified cells were placed in Trizol Solution (GibcoBRL)
|
| Growth protocol |
T cells were isolated from naïve 4-6 week old mice on day 22-35 of chronic LCMV infection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocols for eukaryotic total RNA were used for the samples.All samples were hybed at 45C16 hours60 RPM in 640 Affymetrix Hyb oven. Affymetrix fludics program EUKGEWS2v4_450 was used for washing
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G Plus
|
| Description |
The array used was Murine Genome U74 Version 2 Set MG-U74A. There are 4 groups Naïve Effector Memory and Exhausted . Each group has 3 biological replicates. The biological replicates are numbered 1 to 4; except Effectors 1 to 3 .The
This sample is called Exhausted-2
|
| Data processing |
Genespring
|
| |
|
| Submission date |
Oct 10, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Shruti Subramaniam |
| Organization name |
Emory University
|
| Street address |
Clifton Road
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL82 |
| Series (1) |
| GSE9650 |
Chronic viral infection of naive, effector, memory and exhausted virus-specific CD8 T cells |
|
