|
| Status |
Public on Sep 11, 2017 |
| Title |
L428_scr_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
L428, Negative_Control_siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: L428
perturbagen: Negative_Control_siRNA
|
| Treatment protocol |
L428 were nucleofected with L solution following Lonza optimzalized protocol with either 3ug BATF3siRNA or control siRNA and cultivated for 48 hours.
|
| Growth protocol |
RPMI1640, 10% FCS, 1% pen/strep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed with Qiagen RNeasy Mini Kit
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to PrimeView array using Affymetrix standard protocol
|
| Scan protocol |
Affymetrix Standard protocol Affymetrix Standard protocol
|
| Description |
Gene expression data of L428 cells treated with negative control siRNA
|
| Data processing |
Probe level quantile normalisation and robust multi-array analysis on the raw CEL files were performed using the affy package of the Bioconductor project.
|
| |
|
| Submission date |
Nov 10, 2016 |
| Last update date |
Sep 11, 2017 |
| Contact name |
Wenbin Wei |
| E-mail(s) |
[email protected]
|
| Organization name |
Durham University
|
| Department |
Biosciences
|
| Street address |
Stockton Road
|
| City |
Durham |
| ZIP/Postal code |
DH1 3LE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16043 |
| Series (1) |
| GSE89760 |
BATF3siRNA induced gene expression changes in Hodgkin lymphoma cell line L428. |
|
