|
| Status |
Public on Feb 01, 2017 |
| Title |
wound_control_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse skin wound treated with control mimics
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skin
genotype: db/db [BKS(D)-Leprdb/JOrlRj)]
strain background: C57BL/6
gender: male
|
| Treatment protocol |
0.5 nmol miR-132 mimics or control mimics (ThermoFisher Scientific) were mixed with MaxSuppressor™ In Vivo RNA-LANCEr II (Bioo Scientific) in a total volume of 100 μl for each wound and injected intradermally into 4 points around the wound edges in the mice immediately after wounding. Following the surgical procedure, the animals were individually caged. During the first 2 days, the animals received s.c. buprenorphine (0.03 mg/kg) twice a day for relief of any possible distress caused by the procedure.
|
| Growth protocol |
Eleven-week-old male diabetic db/db [BKS(D)-Leprdb/JOrlRj)] mice on the C57Bl/6J background (Janvier Labs) were caged individually for one week, handled daily, and then wounded. General anesthesia was performed with 3% isoflurane (Abbott). The hair on the back was shaved with an electronic hair remover followed by Veet® hair removal cream. The skin was rinsed with PBS and two full-thickness wounds extending through the panniculus carnosus were made on the dorsum on each side of midline, using a 4 mm biopsy punch.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The mice were sacrificed 6 days after injury and the total RNA was extracted from wound-edge biopsies using miRNeasy Mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
150ng of total RNA were used to prepare cDNA following the GeneChip WT PLUS Reagent Kit labeling protocol.
|
| |
|
| Hybridization protocol |
The samples were hybridized to a mouse Clariom™ D assay plate (previously known as GeneChip® Mouse Transcriptome Assay 1.0) according to standard protocol.
|
| Scan protocol |
The array plate was scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from skin wound treated with control mimics.
|
| Data processing |
Generated CEL files were analyzed using Affymetrix Expression Console v. 1.4 and the SST-RMA method.
|
| |
|
| Submission date |
Dec 14, 2016 |
| Last update date |
Feb 01, 2017 |
| Contact name |
Ning Xu |
| Organization name |
Karolinska Institutet
|
| Street address |
CMM L8:02
|
| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE92409 |
A global transcriptome analysis of skin wounds of diabetic mice (db/db) treated with miR-132 or control mimics |
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