|
| Status |
Public on Sep 05, 2018 |
| Title |
sample6, dataset3 |
| Sample type |
RNA |
| |
|
| Source name |
breast tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast tumor
er status: negative
her2 status: positive
tissue preservation method: Fresh-Frozen
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from frozen samples was extracted using TRIzol® Reagent according to Thermo Fisher Scientific (Invitrogen) recommendations.
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified and labeled using Nugen's Ovation® FFPE WTA System and EncoreTM Biotin Module respectively, according to Nugen's recommendation.
|
| |
|
| Hybridization protocol |
On Affymetrix® HG-U219 Array Plate using GeneTitan® System with GeneTitanTM Hybridization, Wash, and Stain Kit for 3'IVT Array, according to Affymetrix standard procedure.
|
| Scan protocol |
Using GeneTitan® System according to Affymetrix standard procedure.
|
| Data processing |
Data set was RMA background corrected,quantile normalized and median-polish summarized using 'expresso' function available from bioconductor package affy.
|
| |
|
| Submission date |
Jan 09, 2017 |
| Last update date |
Sep 05, 2018 |
| Contact name |
Vinu Jose |
| E-mail(s) |
[email protected]
|
| Organization name |
Institut Jules Bordet – Université Libre de Bruxelles (ULB)
|
| Lab |
Breast Cancer Translational Research Laboratory J.C. Heuson (ULB 290)
|
| Street address |
Rue Meylemeersch 90
|
| City |
Brussels |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL13667 |
| Series (2) |
| GSE93335 |
Fresh-Frozen samples profiled on HG-U219 array with Nugen's Ovation® FFPE WTA System and EncoreTM Biotin Module [dataset 3] |
| GSE93338 |
Feasibility of Developing Reliable Gene Expression Modules from FFPE Derived RNA Profiled on Affymetrix Arrays |
|
