|
| Status |
Public on Jan 10, 2020 |
| Title |
eHCC-10 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse liver tissue
|
| Organism |
Mus musculus |
| Characteristics |
treatment: Diethylnitrosamine (DEN; Sigma) was administered twice by intraperitoneal injections at a dose of 5 mg/kg of body weight at 14 days of age and of 75 mg/kg of body weight at 20 weeks of age.
Stage: eHCC
|
| Treatment protocol |
Diethylnitrosamine (DEN; Sigma) was administered twice by intraperitoneal injections at a dose of 5 mg/kg of body weight at 14 days of age and of 75 mg/kg of body weight at 20 weeks of age. The mice were sacrifice at 15, 20, 36, and 42 weeks after treatment. Blood and liver samples were collected after the mice were sacrificed under ether anesthesia.
|
| Growth protocol |
Male C3H/He N mice were purchased from Central Lab Animal, Inc. (Seoul, Korea), and handled according to the guidelines for the care and use of laboratory animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol Reagent (Invitrogen). RNA quality control was performed with Bio-Rad Experion.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 29
|
| Data processing |
The data were normalized using quantile normalization with GenPlex 3.0
|
| |
|
| Submission date |
Jan 10, 2017 |
| Last update date |
Oct 18, 2022 |
| Contact name |
Jung Woo Eun |
| E-mail(s) |
[email protected]
|
| Organization name |
Ajou University of Korea
|
| Street address |
Department of Gastroenterology
|
| City |
Suwon, Korea |
| State/province |
Korea |
| ZIP/Postal code |
16499 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10242 |
| Series (1) |
| GSE93392 |
Gene-expression profiles of multi-stage hepatocellular carcinoma induced by diethylnitrosamine (DEN) in mouse model |
|
