|
| Status |
Public on Mar 01, 2008 |
| Title |
X20025 |
| Sample type |
RNA |
| |
|
| Source name |
Carcinoma of ovary, fallopian tube or peritonem
|
| Organism |
Homo sapiens |
| Characteristics |
Primary.Site : Peritoneum
Type : Malignant
Subtype : Ser/PapSer
StageCode : IIIC
Consolidated.Grade : 3
|
| Treatment protocol |
Frozen tissue stored at -80oC taken after surgery
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and column chromotogrpahy
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GS300-7G scanner
|
| Description |
Gene expression data from human ovarian carcinoma
|
| Data processing |
The data were analyzed with Gene Chip Operating Software Version 1.4 using Affymetrix default analysis settings. The CEL files generated was later normalized using the RMA method
|
| |
|
| Submission date |
Dec 14, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Prof David Bowtell PhD. |
| E-mail(s) |
[email protected]
|
| Phone |
+613 96561287
|
| Fax |
+613 96561414
|
| URL |
http://www.petermac-research.org.au
|
| Organization name |
PeterMacCallum Cancer Centre
|
| Department |
Research Division
|
| Street address |
Locked Bag 1 A' Beckett St
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
8006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE9891 |
Expression profile of 285 ovarian tumour samples |
| GSE9899 |
Expression profile of ovarian tumour samples |
|
| Relations |
| Reanalyzed by |
GSE40785 |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
