|
| Status |
Public on Jul 05, 2008 |
| Title |
M-05 |
| Sample type |
RNA |
| |
|
| Source name |
Long-term practitioner of daily RR practice
|
| Organism |
Homo sapiens |
| Characteristics |
Effect of MIND and Body on Blood Transcriptome
|
| Treatment protocol |
To measure the Relaxation Response (RR) on Blood transcriptome. The series have data from three sets of subjects: Long RR practitioners Subjects [M], No RR Practice [N1], RR after 8 Weeks of Practice [N2]
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Standard Affymetrix Scanner
|
| Description |
Human Blood
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 28, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Towia A Libermann |
| E-mail(s) |
[email protected]
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Department |
Medicine
|
| Street address |
99 Brookline Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE10041 |
Genomic Counter-Stress Changes Induced by Mind-Body Practice |
|
| Relations |
| Reanalyzed by |
GSE119087 |
