|
| Status |
Public on Sep 14, 2017 |
| Title |
shRNA-H2A.Z.2 rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Resting cortical dissociated neurons, shRNA-H2A.Z.2 shRNA
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: Cortical neurons
shRNA: shRNA-H2A.Z.2
time point: 7 days
|
| Treatment protocol |
Cells were treated with lentiviruses for seven days and RNA was extracted.
|
| Growth protocol |
Cells were grown in Neurobasal media supplemented with B27.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Qiagen RNeasy kit and columns and was DNase digested in column.
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
251487920212-3
Gene expression from neurons treated with shRNA-H2A.Z.2 shRNA.
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v9.5), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Mar 21, 2017 |
| Last update date |
Sep 14, 2017 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4135 |
| Series (1) |
| GSE96886 |
Global effect of H2A.Z hypervariant knockdown on basal expression of neuronal genes |
|
