|
| Status |
Public on May 31, 2017 |
| Title |
EC-Mef2cCdh5EKO-rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Cdh5-CreERT2+; Mef2c f/f mice injected with tamoxicen to delete Mef2c in endothelium
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Thoracic aortic endothelium
Sex: Female
|
| Treatment protocol |
Endothelial-specific, inducible-deletion of Mef2c was mediated by Cdh5-CreERT2 through 5 daily tamoxifen (dissolved in sunflower seed oil with 10% ethanol) injections (Mef2cCdh5EKO) in 10 weeks old mice. Littermates who received vehicle injection (sunflower seed oil with 10% ethanol) served as controls. Thoracic aortas from these mice were harvest 14 days after the first injection.
|
| Growth protocol |
Mice were housed in specific-pathogen free facility.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from TRIzol reagent (ThermoFisher) that flushed through the lumen of individaul mouse thoracic aortas according to manufacturer’s protocol with LPA (Sigma-Aldrich) precipitation.
|
| Label |
Biotin
|
| Label protocol |
5ng total RNA was converted to fragmented biotinylated cDNA using the standard WT Pico protocol (Affymetrix)
|
| |
|
| Hybridization protocol |
labeled samples (5.5ug) were hybridized to Mouse Transcriptome1.0 Array (Affymetrix) using standard protocol
|
| Scan protocol |
The arrays were scanned on a GCS3000 7G scanner using standard Affymetrix protocols
|
| Description |
SST-RMA expression value derived from Expression Console software
|
| Data processing |
The CEL files were converted to CHP files using Expression Consolev 1.4. Further statistical analysis was done using Affymetrix Transcriptome Console v3.0
|
| |
|
| Submission date |
Mar 27, 2017 |
| Last update date |
May 31, 2017 |
| Contact name |
John J Schwarz |
| E-mail(s) |
[email protected]
|
| Organization name |
Albany Medical College
|
| Department |
Department of Molecular and Cellular Physiology
|
| Street address |
47 New Scotland Ave.
|
| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12208 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE97089 |
Transcriptome profiling of murine aortic endothelium deleted for the transcription factor Mef2c |
|
