|
| Status |
Public on Jan 15, 2008 |
| Title |
Mut, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mus musculus embryo E8.5 Tcof1+/-
|
| Organism |
Mus musculus |
| Characteristics |
E.8 DBA-C57BL/6 whole embryo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Protocol for Isolation of Total RNA from Animal Tissues (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix Two-Cycle Target Labeling Protocol from 100 ng total RNA (Expression Analysis Technical Manual, November 2004 update, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hrs at 45C on Mouse Genome 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Mut, biological rep2
|
| Data processing |
The data were analyzed with GCOS (GeneChip Operating Software Version 1.2.0.037) using default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jan 14, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Gaye Hattem |
| E-mail(s) |
[email protected]
|
| Organization name |
Stowers Institute for Medical Research
|
| Department |
Bioinformatics
|
| Street address |
1000 E. 50th Street
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE10167 |
Microarray Analysis of Treacher Collins Syndrome |
|
| Relations |
| Reanalyzed by |
GSE119085 |
