|
| Status |
Public on Mar 01, 2008 |
| Title |
HG- F1 2 Gallus gallus Affymetrix array |
| Sample type |
RNA |
| |
|
| Source name |
HG- Gallus gallus single hen line
|
| Organism |
Gallus gallus |
| Characteristics |
Gallus gallus - age:55 week dev. stage (Boyes et al. Plant Cell 2001)
HG- High growth with less fertile animals
|
| Growth protocol |
ovary - light 14 L _ 10 D - 14h and dark during 10h food : ad libitum aliment standard, pondeuse 5S temperature: 20°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
HG- F1 2:8.8ug. F1 oocytes collected 24 hours before ovulation
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin direct, amplification=yes, RNAa 20 ug.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 15ug
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
DPF+ and DPF- samples were compared in order to find differentially expressed genes that could explain the difference in fertility occuring between the two divergently selected lines. HG+ and HG- samples were compared in order to find differentially expressed genes that could explain the difference in fertility occuring between a single line.
|
| Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
|
| |
|
| Submission date |
Jan 22, 2008 |
| Last update date |
Jan 30, 2008 |
| Contact name |
sandrine balzergue |
| Organization name |
INRA
|
| Lab |
IRHS
|
| Street address |
Rue G. Morel
|
| City |
Beaucouze |
| ZIP/Postal code |
49000 |
| Country |
France |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE10231 |
Gene expression oocyte quality in the hen |
|
