NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM258171 Query DataSets for GSM258171
Status Public on Mar 01, 2008
Title DPF+ F1 2 Gallus gallus Affymetrix array
Sample type RNA
 
Source name DPF+ Gallus gallus divergent hen line
Organism Gallus gallus
Characteristics Gallus gallus - age:55 week dev. stage (Boyes et al. Plant Cell 2001)
DPF+ has a longer fertile period (number of eggs obtained from a single insemination)
Growth protocol ovary - light 14 L _ 10 D - 14h and dark during 10h food : ad libitum aliment standard, pondeuse 5S temperature: 20°C
Extracted molecule total RNA
Extraction protocol DPF+ F1 2:9.1ug. F1 oocytes collected 24 hours before ovulation
Label Biotin
Label protocol labelling Biotin direct, amplification=yes, RNAa 20 ug.
 
Hybridization protocol labelled extract quantity: 15ug
Scan protocol GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
Description DPF+ and DPF- samples were compared in order to find differentially expressed genes that could explain the difference in fertility occuring between the two divergently selected lines. HG+ and HG- samples were compared in order to find differentially expressed genes that could explain the difference in fertility occuring between a single line.
Data processing The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
 
Submission date Jan 22, 2008
Last update date Jan 30, 2008
Contact name sandrine balzergue
Organization name INRA
Lab IRHS
Street address Rue G. Morel
City Beaucouze
ZIP/Postal code 49000
Country France
 
Platform ID GPL3213
Series (1)
GSE10231 Gene expression oocyte quality in the hen

Data table header descriptions
ID_REF ID number
VALUE Normalized data by GCRMA

Data table
ID_REF VALUE
Z46390.CDS2.S1_x_at 3.07
X02759.CDS1.S1_at 2.60
X01455.CDS2.S1_at 2.29
X01455.CDS1.S1_at 2.15
RPTR-Gga-XXU09476-1_at 2.42
RPTR-Gga-X58791-2_s_at 2.32
RPTR-Gga-X58791-1_s_at 2.4
RPTR-Gga-X17220-1_at 2.42
RPTR-Gga-X03453-1_at 2.84
RPTR-Gga-V01555-1_at 2.79
RPTR-Gga-U89963-1_s_at 2.36
RPTR-Gga-U89963-1_at 2.27
RPTR-Gga-U57609-2_s_at 2.15
RPTR-Gga-U55943-1_at 2.81
RPTR-Gga-U47295-1_at 3.11
RPTR-Gga-U46493-1_s_at 2.48
RPTR-Gga-U43284-1_s_at 2.54
RPTR-Gga-NM_131175-1_at 2.08
RPTR-Gga-NC_002086-1_at 2.26
RPTR-Gga-NC_001669-2_at 2.69

Total number of rows: 38535

Table truncated, full table size 931 Kbytes.




Supplementary file Size Download File type/resource
GSM258171.CEL.gz 2.8 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap