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Status |
Public on Mar 01, 2008 |
Title |
DPF+ F1 2 Gallus gallus Affymetrix array |
Sample type |
RNA |
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Source name |
DPF+ Gallus gallus divergent hen line
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Organism |
Gallus gallus |
Characteristics |
Gallus gallus - age:55 week dev. stage (Boyes et al. Plant Cell 2001) DPF+ has a longer fertile period (number of eggs obtained from a single insemination)
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Growth protocol |
ovary - light 14 L _ 10 D - 14h and dark during 10h food : ad libitum aliment standard, pondeuse 5S temperature: 20°C
|
Extracted molecule |
total RNA |
Extraction protocol |
DPF+ F1 2:9.1ug. F1 oocytes collected 24 hours before ovulation
|
Label |
Biotin
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Label protocol |
labelling Biotin direct, amplification=yes, RNAa 20 ug.
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|
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Hybridization protocol |
labelled extract quantity: 15ug
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Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
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Description |
DPF+ and DPF- samples were compared in order to find differentially expressed genes that could explain the difference in fertility occuring between the two divergently selected lines. HG+ and HG- samples were compared in order to find differentially expressed genes that could explain the difference in fertility occuring between a single line.
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Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
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Submission date |
Jan 22, 2008 |
Last update date |
Jan 30, 2008 |
Contact name |
sandrine balzergue |
Organization name |
INRA
|
Lab |
IRHS
|
Street address |
Rue G. Morel
|
City |
Beaucouze |
ZIP/Postal code |
49000 |
Country |
France |
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|
Platform ID |
GPL3213 |
Series (1) |
GSE10231 |
Gene expression oocyte quality in the hen |
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