|
| Status |
Public on Feb 18, 2018 |
| Title |
Low birth weight adult rats on normal diet 1 |
| Sample type |
RNA |
| |
|
| Source name |
Low birth weight adult rats on normal diet
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: male
age of rats (days): 145
birth weight (percentile): 5-25
tissue: Skeletal muscle
|
| Treatment protocol |
On delivery, the pups were weighed and grouped as either of lower (LBW) (5-25th percentile) or average (ABW) (50th-75th percentile) birth weight. Each litter size was adjusted to eight pups per lactating female
|
| Growth protocol |
24 female (~200-210 g) and 12 male (340 to 350 g) ~10-12 week old Wistar rats were mated in 12 groups, each comprising 2 females and a male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from skeletal muscle tissues using TRIzol reagent (Applied Biosystems, Thermo Fisher Scientific, USA) according to manufacturer’s protocol. RNA quality and concentration was determined by using an Agilent 2100 bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed following standard operating protocol from NimbleGen Systems Inc., Madison, WI USA,. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed following standard operating protocol from NimbleGen Systems Inc., Madison, WI, USA. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed following standard operating profocol from NimbleGen Systems Inc., Madison, WI USA. See www.nimblegen.com.
|
| Description |
LBW_AR_ND_1
This sample is from low birth weight (5th-25th percentile) 145-day old adult rats. First of 3 biologial replicates.
|
| Data processing |
The raw data was subjected to RMA (Robust Multi-Array) Analysis and background correction as implemented in the rma package in R.
|
| |
|
| Submission date |
May 12, 2017 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Ranjit Vijayan |
| Organization name |
United Arab Emirates University
|
| Department |
Biology
|
| Street address |
PO Box 15551
|
| City |
Al Ain |
| State/province |
Abu Dhabi |
| ZIP/Postal code |
15551 |
| Country |
United Arab Emirates |
| |
|
| Platform ID |
GPL19519 |
| Series (1) |
| GSE98851 |
Regulatory Mechanisms Mediated by DNA methyltransferase 1 link the maternal Environment with the Development of the Embryo |
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