|
| Status |
Public on Feb 12, 2008 |
| Title |
hMoDC_stim_8h_VAF347_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
immature human monocyte-derived dendritic cells (MoDCs)
|
| Organism |
Homo sapiens |
| Characteristics |
8h, stimulated, treated with VAF347
Donor D2_VAF347
|
| Treatment protocol |
Immature monocyte-derived DC were activated with anti-CD40 antibodies for 8 hours in the absence or presence of 50nM VAF347.
|
| Growth protocol |
Human peripheral blood monocytes were prepared by elutriation or by negative selection of PBMC using a monocyte isolation kit. Monocytes (typically >97% positive for CD14) were differentiated into immature DC by adding 40ng/ml IL-4 and 15ng/ml or 50ng/ml GM-CSF for 6-8 days in the absence or presence of increasing concentrations of VAF347.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Absolutely RNA kit (Stratagene)
|
| Label |
Biotin
|
| Label protocol |
Double-stranded cDNA synthesis was carried out with total RNA (SuperScript double-stranded cDNA synthesis kit; Invitrogen) according to the manufacturer’s instructions.
After purification (QIAquick; Qiagen), the cDNA was transcribed in vitro in the presence of biotinylated ribonucleotides (Bioarray High yield T7 DNA transcription kit, ENZO Life Sciences Inc.). The labeled cRNA was purified on an affinity resin (RNeasy, Qiagen), quantified and fragmented into strands of approximately 50-200 nucleotides in length.
|
| |
|
| Hybridization protocol |
Hybridization was carried out at 45ºC for approximately 18 hr.
|
| Scan protocol |
The arrays were stained on a GeneChip Fluidics Workstation 450 and scanned on GeneArray Scanner 3000 according to manufacturer’s technical manual (Affymetrix).
|
| Description |
na
|
| Data processing |
The obtained signal intensities (.CEL files) were uploaded into GeneSpring (Agilent Technologies)
|
| |
|
| Submission date |
Feb 11, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Meinrad Busslinger |
| E-mail(s) |
[email protected]
|
| Organization name |
IMP
|
| Lab |
Busslinger
|
| Street address |
Campus-Vienna-Biocenter 1
|
| City |
Vienna |
| ZIP/Postal code |
A-1030 |
| Country |
Austria |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE10463 |
Activation of aryl hydrocarbon receptor |
|
| Relations |
| Reanalyzed by |
GSE49910 |
| Reanalyzed by |
GSE119087 |
