|
| Status |
Public on Aug 11, 2017 |
| Title |
JU1580DRH1_inf3 |
| Sample type |
RNA |
| |
|
| Source name |
JU1580+N2drh1 Orsay infected
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: JU1580_rescuedrh1
infected with: Orsay
|
| Treatment protocol |
Infection with Orsay virus for 4 days at 20C
|
| Growth protocol |
C.elegans were grown under standard conditions at 20C The food source used was E. coli strain HB101 (Caenorhabditis Genetics Center, University of Minnesota, Twin Cities, MN, USA). Uninfected animals were picked as L4s. Virus or control solution was added after 24 hrs and RNA was harvested 4 days later
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol
|
| Label |
Biotin
|
| Label protocol |
labelling, hybridisation and scanning was carried out by the University of Cambridge Institute of Metabolic Sciences microarray facility
|
| |
|
| Hybridization protocol |
labelling, hybridisation and scanning was carried out by the University of Cambridge Institute of Metabolic Sciences microarray facility
|
| Scan protocol |
labelling, hybridisation and scanning was carried out by the University of Cambridge Institute of Metabolic Sciences microarray facility
|
| Data processing |
The Bioconductor package was used to generate RMA normalized values for each probe [R ver 2.14 and Bioconductor ver 2.10, package affy].
|
| |
|
| Submission date |
Jun 01, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Eric Alexander Miska |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Cambridge
|
| Department |
Gurdon Institute
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19230 |
| Series (1) |
| GSE99586 |
Role of DRH1 in C. elegans virus infection gene expression response |
|
