|
| Status |
Public on Mar 10, 2020 |
| Title |
Myb_mutant_biol_rep2_chip1 |
| Sample type |
RNA |
| |
|
| Source name |
Wing discs of Myb mutant 3rd instar larvae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: Myb^MH107: "mutation induced by P-element mobilization by Delta2-3 transposase"
phenotype: Lethal-early pupal stage
gender: Male
developmental stage: 3rd instar larvae
tissue: Imaginal wing disc
|
| Treatment protocol |
3rd instar larvae were dissected in 1X PBS (pH 7.4)
|
| Growth protocol |
Flies were mantined at 25°C in malt-based media (Archon Scientific).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIzol (Invitrogen) and purified with RNeasy Mini Kit (Qiagen). Double-stranded cDNA was generated using Double-Stranded cDNA Synthesis Kit with random hexamer (Invitrogen). RNA and cDNA quality were analyzed using Experion RNA and DNA analysis kits (Bio-Rad), respectively.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed following their standard protocol. See www.roche.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed following their standard protocol. See www.roche.com.
|
| Scan protocol |
Scanning was performed following their standard protocol. See www.roche.com.
|
| Description |
Myb mutant imaginal wing discs of 3rd instar larvae. Chip one of the second biological replicate
SAMPLE 2
|
| Data processing |
Raw data (.pair files) were run in NimbleScan to produce .gff files
Signal intensities of individual probes corresponding to specific genomic locations.
|
| |
|
| Submission date |
Jun 16, 2017 |
| Last update date |
Mar 11, 2020 |
| Contact name |
David H Price |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Iowa
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
260 EMRB
|
| Street address |
500 Newton Road
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL23595 |
| Series (2) |
| GSE100141 |
Gene expression profiling of Drosophila melangoaster Myb mutant wing discs of 3rd instar larvae. [NimbleGen tiling array] |
| GSE100143 |
Drosophila melangoaster Myb mutant wing discs of 3rd instar larvae. |
|
